Development of animal model for Bisphosphonates-related osteonecrosis of the jaw (BRONJ)
장효원, 김진우, 차인호,
소속 상세정보
장효원 ( Jang Hyo-Won ) - Yonsei University College of Dentistry Department of Oral and Maxillofacial Surgery
김진우 ( Kim Jin-Woo ) - Ewha Womans University Mokdong Hospital Department of Oral and Maxillofacial Surgery
차인호 ( Cha In-Ho ) - Yonsei University College of Dentistry Department of Oral and Maxillofacial Surgery
Abstract
Background: The aim of this study is to develop a rat model of bisphosphonates-related osteonecrosis of the jaw (BRONJ) that would be verified with clinical, radiological and histological examination, and to confirm the influence of concurrent bisphosphonates and steroids use upon the occurrence and aggravation of BRONJ.
Methods: Twenty seven rats were divided into 3 groups; Saline group (I), Zoledronate group (II), Zoledronate and Dexamethasone group (III). Rats got weekly intraperitoneal injection for 4 times and extraction of left maxillary and mandibular 1st, 2nd molars were followed. Consecutive injections were performed, and blood sampling for measurements of C-terminal crosslinked telopeptide of type I collagen and tartrate-resistant acid phosphate 5b rats were performed at the time of 2, 4 and 8 weeks. And then, rats were sacrificed and evaluated clinically, radiologically and histologically.
Results: 12/18 (66.6 %) of experimental group were diagnosed as BRONJ. There was no significant difference in incidence between zoledronate alone group (ll) and concurrent use of zoledronate and dexamethasone group (lll).
Conclusions: Concurrent use of bisphosphonates and steroids increase incidence of BRONJ compared to saline group (l). Zoledronate alone group (ll) and concurrent use of zoledronate and dexamethasone group (lll) shows same incidence of BRONJ. Based on this study, the rat treated with bisphosphonates and steroids can be considered a novel, reliable and reproducible model to understand pathology of BRONJ.
키워드
Bisphosphonates; Osteonecrosis of the jaw; Steroid; Rat model
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